| 质粒名称: | pDONR207 SARS-CoV-2 NSP3 |
|---|---|
| 出品公司: | 爱迪基因 |
| 目录编号: | 141257 |
| 存储实验室: | Fritz Roth |
|
载体骨架基本信息 |
|
| 载体名称: | pDONR207 |
| 载体出品公司: | Invitrogen |
| 载体抗性: | 庆大霉素 |
| 空载体大小: | 5585 bp |
| 完整质粒大小: | -- |
| 载体修饰: | -- |
| 载体类型: | Gateway-compatible Entry vector |
| 筛选标记: | -- |
| 高拷贝/低拷贝: | -- |
| 生长条件: | 37 ℃ |
|
插入基因信息 |
|
| 插入基因名称: | NSP3,PLPRO,Gene ID 43740578 |
| 物种来源: | SARS-CoV-2 isolate Wuhan-Hu-1 |
| 插入大小: | 5841 bp |
| 基因突变: | Many synonymous changes due to codon optimization |
| GenBank编号 | -- |
| 启动子 | -- |
| 标签/融合蛋白: | -- |
| 克隆方法: | -- |
| 5' 克隆位点: | -- |
| 3' 克隆位点: | -- |
| 5' 测序引物 | -- |
| 3' 测序引物 | -- |
|
文献引用方法 |
|
| 材料和方法部分: | pDONR207 SARS-CoV-2 NSP3 was a gift from Fritz Roth (Addgene plasmid # 141257 ; http://n2t.net/addgene:141257 ; RRID:Addgene_141257) |
| 参考文献部分: | A Comprehensive, Flexible Collection of SARS-CoV-2 Coding Regions. Kim DK, Knapp JJ, Kuang D, Chawla A, Cassonnet P, Lee H, Sheykhkarimli D, Samavarchi-Tehrani P, Abdouni H, Rayhan A, Li R, Pogoutse O, Coyaud E, van der Werf S, Demeret C, Gingras AC, Taipale M, Raught B, Jacob Y, Roth FP. G3 (Bethesda). 2020 Aug 6. pii: g3.120.401554. doi: 10.1534/g3.120.401554. 10.1534/g3.120.401554 PubMed 32763951 |
买家导航
载体基本信息
载体图谱质粒图谱和多克隆位点信息
载体简介
载体序列
LOCUS pDONR207 SARS-CoV-2 NSP3 9211 bp ds-DNA circular SYN 12-MAY-2021
DEFINITION Gateway-compatible Entry vector, with insert of NSP3 gene's CDS from
SARS-CoV-2 isolate Wuhan-Hu-1.
ACCESSION .
VERSION .
KEYWORDS pDONR207 SARS-CoV-2 NSP3
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 9211)
AUTHORS Kim DK, Knapp JJ, Kuang D, Chawla A, Cassonnet P, Lee H,
Sheykhkarimli D, Samavarchi-Tehrani P, Abdouni H, Rayhan A, Li R,
Pogoutse O, Coyaud E, van der Werf S, Demeret C, Gingras AC, Taipale
M, Raught B, Jacob Y, Roth FP
TITLE A Comprehensive, Flexible Collection of SARS-CoV-2 Coding Regions.
JOURNAL G3 (Bethesda). 2020 Aug 6. pii: g3.120.401554. doi:
10.1534/g3.120.401554.
PUBMED 32763951
REFERENCE 2 (bases 1 to 9211)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported May 12, 2021 from SnapGene Server 1.1.58
http://www.snapgene.com
FEATURES Location/Qualifiers
source 1..9211
/organism="synthetic DNA construct"
/mol_type="other DNA"
terminator 56..83
/label=rrnB T2 terminator
/note="transcription terminator T2 from the E. coli rrnB
gene"
terminator 175..261
/gene="Escherichia coli rrnB"
/label=rrnB T1 terminator
/note="transcription terminator T1 from the E. coli rrnB
gene"
protein_bind 314..413
/gene="mutant version of attL"
/label=attL1
/bound_moiety="LR Clonase(TM)"
/note="recombination site for the Gateway(R) LR reaction"
protein_bind complement(6255..6354)
/gene="mutant version of attL"
/label=attL2
/bound_moiety="LR Clonase(TM)"
/note="recombination site for the Gateway(R) LR reaction"
primer_bind complement(6466..6485)
/label=pENTR-R
/note="pENTR vectors, reverse primer"
primer_bind complement(6544..6563)
/label=Kan-R
/note="Kanamycin resistance gene, reverse primer"
CDS complement(7137..7670)
/codon_start=1
/gene="aacC1"
/product="gentamycin acetyltransferase"
/label=GmR
/note="confers resistance to gentamycin"
/translation="MLRSSNDVTQQGSRPKTKLGGSSMGIIRTCRLGPDQVKSMRAALD
LFGREFGDVATYSQHQPDSDYLGNLLRSKTFIALAAFDQEAVVGALAAYVLPKFEQPRS
EIYIYDLAVSGEHRRQGIATALINLLKHEANALGAYVIYVQADYGDDPAVALYTKLGIR
EEVMHFDIDPSTAT"
promoter complement(7859..7887)
/gene="intI1 (promoter lies within the coding sequence)"
/label=Pc promoter
/note="class 1 integron promoter"
rep_origin 8544..9132
/direction=RIGHT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
primer_bind 9033..9052
/label=pBR322ori-F
/note="pBR322 origin, forward primer"
ORIGIN
1 ctgattctgt ggataaccgt attaccgcta gccaggaaga gtttgtagaa acgcaaaaag
61 gccatccgtc aggatggcct tctgcttagt ttgatgcctg gcagtttatg gcgggcgtcc
121 tgcccgccac cctccgggcc gttgcttcac aacgttcaaa tccgctcccg gcggatttgt
181 cctactcagg agagcgttca ccgacaaaca acagataaaa cgaaaggccc agtcttccga
241 ctgagccttt cgttttattt gatgcctggc agttccctac tctcgcgtta acgctagcat
301 ggatctcggg ccccaaataa tgattttatt ttgactgata gtgacctgtt cgttgcaaca
361 aattgatgag caatgctttt ttataatgcc aactttgtac aaaaaagttg gcatggcccc
421 taccaaggtg accttcggag acgacaccgt gatcgaggtg cagggttaca agagcgtgaa
481 catcaccttc gagctggacg aacgcatcga caaggtgctg aacgaaaagt gctccgctta
541 caccgtggag ctgggcaccg aggtgaacga attcgcctgc gtggtggccg acgctgtgat
601 caagaccctg cagccagtgt ccgaactgct gacccctctg ggtatcgacc tggacgagtg
661 gtctatggct acctactacc tgttcgacga gagcggcgaa ttcaagctgg cctctcacat
721 gtactgcagc ttctaccctc ccgacgaaga cgaggaagag ggtgactgcg aagaggaaga
781 gttcgagcct tccactcagt acgagtacgg caccgaagac gactaccagg gcaagcctct
841 ggaattcgga gccacctctg ccgctctgca gcctgaagag gaacaggagg aagactggct
901 ggacgacgac tcccagcaga ccgtgggtca gcaggacggc tctgaagaca accagaccac
961 caccatccag accatcgtgg aggtgcagcc tcagctggag atggaactga ccccagtggt
1021 gcagaccatc gaggtgaaca gcttctccgg ctacctgaag ctgaccgaca acgtgtacat
1081 caagaacgct gacatcgtgg aggaagccaa gaaggtgaaa ccaaccgtgg tggtgaacgc
1141 cgctaacgtg tacctgaagc acggcggagg tgtggctgga gctctgaaca aggctaccaa
1201 caacgccatg caggtggaat ccgacgacta catcgctacc aacggtcctc tgaaggtggg
1261 cggatcctgc gtgctgtctg gccacaacct ggccaagcac tgcctgcacg tggtgggacc
1321 aaacgtgaac aagggagagg acatccagct gctgaagagc gcctacgaaa acttcaacca
1381 gcacgaggtg ctgctggctc cactgctgtc tgccggaatc ttcggtgctg atcccatcca
1441 cagcctgcgc gtgtgcgtgg acaccgtgag gaccaacgtg tacctggccg tgttcgacaa
1501 gaacctgtac gacaagctgg tgagctcctt cctggagatg aagtccgaaa agcaggtgga
1561 gcagaagatc gccgagatcc ccaaggagga agtgaagcct ttcatcaccg aatctaagcc
1621 cagcgtggag cagagaaagc aggacgacaa gaagatcaag gcctgcgtgg aggaagtgac
1681 caccaccctg gaggaaacca agttcctgac cgagaacctg ctgctgtaca tcgacatcaa
1741 cggcaacctg caccctgact ccgctaccct ggtgtctgac atcgacatca ccttcctgaa
1801 gaaggacgcc ccctacatcg tgggcgacgt ggtgcaggaa ggagtgctga ccgctgtggt
1861 catccctacc aagaaggccg gtggcaccac cgagatgctg gccaaggctc tgcgtaaggt
1921 gccaaccgac aactacatca ccacctaccc cggtcagggc ctgaacggtt acaccgtgga
1981 ggaagctaag accgtgctga agaagtgcaa gtctgccttc tacatcctgc cctccatcat
2041 ctctaacgag aagcaggaaa tcctgggcac cgtgagctgg aacctgcgcg aaatgctggc
2101 ccacgctgag gaaaccagga agctgatgcc cgtgtgcgtg gagaccaagg ccatcgtgag
2161 caccatccag agaaagtaca agggcatcaa gatccaggag ggtgtggtgg actacggcgc
2221 tcgtttctac ttctacacct ccaagaccac cgtggcctct ctgatcaaca ccctgaacga
2281 cctgaacgag accctggtga ccatgccact gggatacgtg acccacggtc tgaacctgga
2341 ggaagccgct agatacatgc gttccctgaa ggtgcctgct accgtgagcg tgagcagccc
2401 tgacgctgtg accgcctaca acggatacct gacctcctct agcaagaccc ctgaggaaca
2461 cttcatcgaa accatcagcc tggccggttc ctacaaggac tggtcttaca gcggacagtc
2521 cacccagctg ggcatcgagt tcctgaagcg cggagacaag agcgtgtact acacctccaa
2581 cccaaccacc ttccacctgg acggtgaagt gatcaccttc gacaacctga agaccctgct
2641 gtctctgcgc gaggtgagga ccatcaaggt gttcaccacc gtggacaaca tcaacctgca
2701 cacccaggtg gtggacatga gcatgaccta cggacagcag ttcggtccta cctacctgga
2761 cggagctgac gtgaccaaga tcaagccaca caactcccac gagggcaaga ccttctacgt
2821 gctgcctaac gatgacaccc tgagagtgga ggctttcgaa tactaccaca ccaccgaccc
2881 atccttcctg ggcaggtaca tgtctgccct gaaccacacc aagaagtgga agtaccccca
2941 ggtgaacgga ctgaccagca tcaagtgggc cgacaacaac tgctacctgg ccaccgctct
3001 gctgaccctg cagcagatcg aactgaagtt caacccaccc gctctgcagg acgcttacta
3061 cagagcccgt gctggcgagg ccgctaactt ctgcgctctg atcctggcct actgcaacaa
3121 gaccgtgggc gaactgggag acgtgagaga gaccatgagc tacctgttcc agcacgccaa
3181 cctggactcc tgcaagcgtg tgctgaacgt ggtgtgcaag acctgcggcc agcagcagac
3241 caccctgaag ggagtggaag ctgtgatgta catgggtacc ctgtcctacg agcagttcaa
3301 gaagggcgtg cagatccctt gcacctgcgg aaagcaggcc accaagtacc tggtgcagca
3361 ggaaagccca ttcgtgatga tgtccgctcc tccagcccag tacgaactga agcacggcac
3421 cttcacctgc gcttccgagt acaccggcaa ctaccagtgc ggacactaca agcacatcac
3481 ctctaaggag accctgtact gcatcgacgg agccctgctg accaagtcct ctgaatacaa
3541 gggtcccatc accgacgtgt tctacaagga gaacagctac accaccacca tcaagcccgt
3601 gacctacaag ctggacggcg tggtgtgcac cgaaatcgac cctaagctgg acaactacta
3661 caagaaggac aactcttact tcaccgagca gcctatcgac ctggtgccaa accagcccta
3721 ccctaacgcc agcttcgaca acttcaagtt cgtgtgcgac aacatcaagt tcgctgacga
3781 cctgaaccag ctgaccggat acaagaagcc tgcctctaga gagctgaagg tgaccttctt
3841 cccagacctg aacggcgacg tggtggctat cgactacaag cactacaccc caagcttcaa
3901 gaagggagcc aagctgctgc acaagcccat cgtgtggcac gtgaacaacg ctaccaacaa
3961 ggccacctac aagcctaaca cctggtgcat ccgttgcctg tggtctacca agccagtgga
4021 aacctccaac tctttcgacg tgctgaagag cgaggacgct cagggcatgg acaacctggc
4081 ctgcgaagac ctgaagcccg tgagcgagga agtggtggag aacccaacca tccagaagga
4141 cgtgctggaa tgcaacgtga agaccaccga ggtggtggga gacatcatcc tgaagccagc
4201 taacaacagc ctgaagatca ccgaggaagt gggtcacacc gacctgatgg ccgcttacgt
4261 ggacaacagc tccctgacca tcaagaagcc caacgagctg agcagggtgc tgggtctgaa
4321 gaccctggct actcacggtc tggctgctgt gaactctgtg ccatgggaca ccatcgctaa
4381 ctacgccaag cctttcctga acaaggtggt gtccaccacc accaacatcg tgaccagatg
4441 tctgaaccgc gtgtgcacca actacatgcc ctacttcttc accctgctgc tgcagctgtg
4501 caccttcacc agaagcacca actcccgtat caaggctagc atgcctacca ccatcgccaa
4561 gaacaccgtg aagagcgtgg gaaagttctg cctggaggct tccttcaact acctgaagtc
4621 tccaaacttc agcaagctga tcaacatcat catctggttc ctgctgctct ccgtgtgcct
4681 gggttccctg atctactcta ccgccgctct gggagtgctg atgtctaacc tgggtatgcc
4741 tagctactgc actggttaca gagagggata cctgaacagc accaacgtga ccatcgccac
4801 ctactgcacc ggcagcatcc catgctccgt gtgcctgtct ggactggaca gcctggacac
4861 ctacccctcc ctggaaacca tccagatcac catctctagc ttcaagtggg acctgaccgc
4921 cttcggtctg gtggctgagt ggttcctggc ctacatcctg ttcaccaggt tcttctacgt
4981 gctgggcctg gccgctatca tgcagctgtt cttctcctac ttcgctgtgc acttcatcag
5041 caactcctgg ctgatgtggc tgatcatcaa cctggtgcag atggctccca tctctgccat
5101 ggtgcgcatg tacatcttct tcgccagctt ctactacgtg tggaagtcct acgtgcacgt
5161 ggtggacggt tgcaactcct ctacctgcat gatgtgctac aagcgcaaca gggctaccag
5221 ggtggagtgc accaccatcg tgaacggcgt gcgcaggtcc ttctacgtgt acgccaacgg
5281 cggcaaggga ttctgcaagc tgcacaactg gaactgcgtg aactgcgaca ccttctgcgc
5341 tggctccacc ttcatctctg acgaggtggc cagagacctg agcctgcagt tcaagcgtcc
5401 aatcaacccc accgaccaga gctcctacat cgtggactct gtgaccgtga agaacggtag
5461 catccacctg tacttcgaca aggctggcca gaagacctac gagcgccact ctctgagcca
5521 cttcgtgaac ctggacaacc tgagggccaa caacaccaag ggcagcctgc ctatcaacgt
5581 gatcgtgttc gacggaaagt ccaagtgcga ggaatctagc gccaagtccg ccagcgtgta
5641 ctacagccag ctgatgtgcc agccaatcct gctgctggac caggctctgg tgtctgacgt
5701 gggagacagc gccgaagtgg ccgtgaagat gttcgacgcc tacgtgaaca ccttctcctc
5761 taccttcaac gtgcctatgg agaagctgaa gaccctggtg gctactgctg aggctgaact
5821 ggctaagaac gtgtccctgg acaacgtgct gagcaccttc atctccgccg ctaggcaggg
5881 tttcgtggac tctgacgtgg aaaccaaaga cgtggtggag tgcctgaagc tgtctcacca
5941 gagcgacatc gaagtgaccg gcgactcctg caacaactac atgctgacct acaacaaggt
6001 ggagaacatg accccaagag acctgggagc ttgcatcgac tgctccgccc gtcacatcaa
6061 cgctcaggtg gccaagtctc acaacatcgc cctgatctgg aacgtgaagg acttcatgag
6121 cctgtccgag cagctgcgca agcagatcag gagcgccgct aagaagaaca acctgccctt
6181 caagctgacc tgcgctacca ccagacaggt ggtgaacgtg gtgaccacca agatcgccct
6241 gaagggcgga taatacccaa ctttcttgta caaagttggc attataagaa agcattgctt
6301 atcaatttgt tgcaacgaac aggtcactat cagtcaaaat aaaatcatta tttgccatcc
6361 agctgcagct ctggcccgtg tctcaaaatc tctgatgtta cattgcacaa gataaaaata
6421 tatcatcatg aacaataaaa ctgtctgctt acataaacag taatacaagg ggtgttatga
6481 gccatattca acgggaaacg tcgaggccgc gattaaattc caacatggat gctgatttat
6541 atgggtataa atgggctcgc gataatgtcg ggcaatcagg tgcgacaatc tatcgcttgt
6601 atgggaagcc cgatgcgcca gagttgtttc tgaaacatgg caaaggtagc gttgccaatg
6661 atgttacaga tgagatggtc agactaaact ggctgacgga atttatgcct cttccgacca
6721 tcaagcattt tatccgtact cctgatgatg catggttact caccactgcg atccccggaa
6781 aaacagcatt ccaggtatta gaagaatatc ctgattcagg tgaaaatatt gttgatgcgc
6841 tggcagtgtt cctgcgccgg ttgcattcga ttcctgtttg taattgtcct tttaacagcg
6901 atcgcgtatt tcgtctcgct caggcgcaat cacgaatgaa taacggtttg gttgatgcga
6961 gtgattttga tgacgagcgt aatggctggc ctgttgaaca agtctggaaa gaaatgcata
7021 aacttttgcc attctcaccg gattcagtcg tcactcatgg tgatttctca cttgataacc
7081 ttatttttga cgaggggaaa ttaggccggg aagccgatct cggcttgaac gaattgttag
7141 gtggcggtac ttgggtcgat atcaaagtgc atcacttctt cccgtatgcc caactttgta
7201 tagagagcca ctgcgggatc gtcaccgtaa tctgcttgca cgtagatcac ataagcacca
7261 agcgcgttgg cctcatgctt gaggagattg atgagcgcgg tggcaatgcc ctgcctccgg
7321 tgctcgccgg agactgcgag atcatagata tagatctcac tacgcggctg ctcaaacttg
7381 ggcagaacgt aagccgcgag agcgccaaca accgcttctt ggtcgaaggc agcaagcgcg
7441 atgaatgtct tactacggag caagttcccg aggtaatcgg agtccggctg atgttgggag
7501 taggtggcta cgtctccgaa ctcacgaccg aaaagatcaa gagcagcccg catggatttg
7561 acttggtcag ggccgagcct acatgtgcga atgatgccca tacttgagcc acctaacttt
7621 gttttagggc gactgccctg ctgcgtaaca tcgttgctgc tgcgtaacat cgttgctgct
7681 ccataacatc aaacatcgac ccacggcgta acgcgcttgc tgcttggatg cccgaggcat
7741 agactgtaca aaaaaacagt cataacaagc catgaaaacc gccactgcgc cgttaccacc
7801 gctgcgttcg gtcaaggttc tggaccagtt gcgtgagcgc atacgctact tgcattacag
7861 tttacgaacc gaacaggctt atgtcaactg ggttcgtgcc ttcatccgtt tccacggtgt
7921 gcgtcacccg gcaaccttgg gcagcagcga agtcgaggca tttctgtcct ggctggcgaa
7981 cgagcgcaag gtttcggtct ccacgcatcg tcaggcattg gcggccttgc tgttcttcta
8041 cggcaaggtg ctgtgcacgg atctgccctg gcttcaggag atcggaagac ctcggccgtc
8101 gcggcgcttg ccggtggtgc tgaccccgga tgaagtggtt cgcatcctcg gttttctgga
8161 aggcgagcat cgtttgttcg cccaggactc tagctatagt tctagtggtt ggctactaat
8221 aggttgtatt gatgttggac gagtcggaat cgcagaccga taccaggatc ttgccatcct
8281 atggaactgc ctcggtgagt tttctccttc attacagaaa cggctttttc aaaaatatgg
8341 tattgataat cctgatatga ataaattgca gtttcatttg atgctcgatg agtttttcta
8401 atcagaattg gttaattggt tgtaacactg gcagagcatt acgctgactt gacgggacgg
8461 cgcaagctca tgaccaaaat cccttaacgt gagttttcgt tccactgagc gtcagacccc
8521 gtagaaaaga tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg
8581 caaacaaaaa aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact
8641 ctttttccga aggtaactgg cttcagcaga gcgcagatac caaatactgt tcttctagtg
8701 tagccgtagt taggccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg
8761 ctaatcctgt taccagtggc tgctgccagt ggcgataagt cgtgtcttac cgggttggac
8821 tcaagacgat agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca
8881 cagcccagct tggagcgaac gacctacacc gaactgagat acctacagcg tgagctatga
8941 gaaagcgcca cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc
9001 ggaacaggag agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct
9061 gtcgggtttc gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg
9121 agcctatgga aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct
9181 tttgctcaca tgttctttcc tgcgttatcc c
//
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